The Preclinical Imaging (PCI), as an integral part of the IU Department of Radiology and Imaging Sciences, has been created to enhance the preclinical, translational or clinical research by novel in vivo imaging techniques. The PCI provides comprehensive imaging support services for investigators, both intramural and extramural, and provide biomarkers that can help facilitate translation of your experimental therapy from animal models to humans.
Functional and anatomical small animal imaging on Siemens MAGNETOM Trio 3T MRI with dedicated animal RF coils
IndyPETIII scanner, designed to achieve 1 µL volumetric spatial resolution suitable for whole body mouse imaging
The EVS-R9 microCT scanner, capable of 50 µm or 100 µm voxel resolution with 2x2 or 4x4 binning in the detector panel.
Imaging luciferase and fluorescent protein expression, as well as imaging fluorescent probes in small animals.
The preclinical imaging Lab has a very wide array of animal imaging capabilities that include: MRI, PET/CT, CT and Optical (BLI, FLI) imaging which provide a unique opportunity to permit testing, validation and in vivo verification at a single site.This laboratory is located in the Research Institute II (R2) building which is centrally located within the Medical School campus. The first floor and basement level of the R2 building house these animal imaging facilities along with ancillary support space including wet laboratories, surgery suites, an image processing laboratory, a machine shop, and an electronics shop. The imaging center is housed within approximately 30,000 GSF space.
A second building, the Biomedical Research and Training Center (BRTC), houses our tracer and contrast agent development program. This space can accommodate 2 medical cyclotrons (1 is currently located in the facility) and has extensive synthetic chemistry space, radiochemistry space, molecular biology laboratories, cell culture labs, and ancillary support labs for shared instrumentation and device construction.
Preclinical imaging is the visualization of living animals for research purposes. Imaging modalities have long been crucial to the researcher in observing changes, either at the organ, tissue, cell, or molecular level, in animals responding to physiological or environmental changes.
The most suitable modalities for small-animal in vivo imaging applications are based on magnetic resonance imaging (MRI), positron emission tomography (PET), optical imaging (OI), computed tomography (CT), and ultrasound, etc.
The preclinical imaging lab provides experienced and trained technologist for the acquisition, image processing, and analysis required for all studies. Investigators, or their staff, work closely with the preclinical imaging lab technologists in the performance of specific studies. The preclinical imaging lab has both certified animal and imaging technologist capable of administering anesthesia, PET tracers or contrast agents, and operate the imaging systems. The preclinical imaging staff is also responsible for all necessary image reconstruction or processing needed for the study. An emphasis for all imaging studies is placed on the accurate and reproducible collection of data and image analysis that permits quantitative, or semi-quantitative, analysis of results.
The preclinical imaging lab also maintains numerous servers and software packages for the analysis of imaging data. A large base of in-house developed (based upon, .Net, IDL and/or MATLAB) and commercial (Analyze, AnalyzeDirect) image processing and analysis software is supported.